Unveiling Facet-Based DNA Polyhedra with Cryogenic Electron Microscopy

Project Summary: We have designed multiple platonic and Kepler-Poinsot polyhedra using single-stranded DNAs with a facetbased method that we developed in HKU. Research found that the increase in spikiness of nanoparticle could enhance the cell entry for drug delivery. The successful observation of our DNA polyhedra will create a solid foundation for the later stages of research. The traditional microscopies including transmission electron microscopy (TEM) and atomic force microscopy (AFM) could not provide high enough resolution to reveal the details of nanostructures. With the newly established Cryogenic Electron Microscopy (Cryo-EM) at the faculty of medicine, we have already started to observe structural details. We wish to offer an opportunity for undergraduates engaging in this frontier research of the faculty. Objectives of this project are as follows:
1. Validate the assembly of platonic and Kepler-Poinsot DNA polyhedra
2. Optimize the condition of assembly at high concentration for Cryo-EM
3. Observe and reconstruct the 3D structure of DNA polyhedra with microscopic technique including TEM, AFM and Cryo-EM

Deliverables: Students are expected to actively participate in the project with experimental observations and data analysis. This innovative project is expected to be published in high impact journals. Students could also present their observations in conferences.

Preferred discipline(s): DNA nanotechnology is a highly multidisciplinary research field. Students from any science or engineering related program will be considered.

Project Essential Skills:

• Time management
• Basic knowledge on DNA structures is preferred
• Prior knowledge or experience in biochemical techniques is preferred such as gel electrophoresis

Other Selection Criteria (if any): This project requires a lot of dedication. Students will be selected based on their passion and willingness of dedicating their time.

Details of supervision arrangements: This project requires involvement in hands-on research in the laboratory. There will be daily discussion with mentor on the experiments and weekly discussion with supervisors on the research progress.